PSX-10 - Genome-wide association studies for clinical mastitis in dairy cattle

Wednesday, July 24, 2024

9:45 AM – 10:45 AM MT

Location: Exhibit Hall CD

Author(s)

ZC

Zikai K. Chen

Graduate Student
Department of Animal Science, California Polytechnic State University
San Luis Obispo, California, United States
DS

Desiree Seto

Graduate Student
Animal Science Dept, California Polytechnic State University
San Luis Obispo, California, United States

Author(s)

FC

Fernando Campos

Professor, Veterinarian
Department of Animal Science, California Polytechnic State University
San Luis Obispo, California, United States
JH

Julie M. Huzzey

Department of Animal Science, California Polytechnic State University
San Luis Obispo, California, United States
DP

Dan Peterson

Professor, Molecular Physiology and Genomics Specialist
Department of Animal Science, California Polytechnic State University
San Luis Obispo, California, United States
KS

Kim Sprayberry

Associate Professor, Veterinarian
Department of Animal Science, California Polytechnic State University
San Luis Obispo, California, United States
MS

Mehdi Sargolzaei

Department of Pathobiology, University of Guelph
Guelph, Ontario, Canada
PA

Paul Anderson

Associate Professor
Department of Animal Science, California Polytechnic State University
San Luis Obispo, California, United States
JA

Joy Altermatt

Department of Animal Science, California Polytechnic State University
San Luis Obispo, California, United States
BG

Bruce Golden

Department of Animal Science, California Polytechnic State University
San Luis Obispo, California, United States
SP

Siroj Pokharel

Assistant Professor
Department of Animal Science, California Polytechnic State University
San Luis Obispo, California, United States

Presenting Author(s)

Mohammed Abo-Ismail, PhD (he/him/his)

Assistant Professor
Department of Animal Science, California Polytechnic State University
San Luis Obispo, California, United States

Abstract: Mastitis, the inflammation of the mammary gland caused by both gram-positive and gram-negative bacteria, causes the global dairy industry over $20 billion annually in losses due to changes in milk yield and quality. The goal of this study was to identify genetic variants, genes, and biological pathways that are associated with mastitis. We performed genome-wide association studies (GWAS) on 1,858 cows from two lactations with both medium (n=87,493) and high density (n=624,300) single nucleotide polymorphisms (SNP) using a single SNP mixed linear model implemented in GCTA software. A total of 288 SNPs across all evaluated models passed the 5% genome-wise false discovery rate threshold across all autosomes with emphasis on chromosomes 3, 14, 16, 12, 6, and 2. These SNPs were mapped to their corresponding genes using the bovine genome. Gene enrichment analysis was then performed to identify significant biological pathways associated with mastitis. The current study identified potential genes that are involved in the MAPK, C5a, and ion binding pathways, which have been previously associated with immune function and inflammation. Further validation and investigation is required to confirm the identified genes and pathways before implementation in breeding programs.